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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has actually been noticed in many tumors, which ends up in chromosome instability, and therefore could contribute to tumorigenesis. This protein was discovered to affiliate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a essential purpose in advertising and marketing cell-cycle development from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are described. Two additional splice variants were being described but detailed nucleotide sequence information and facts will not be however available.
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Blocking peptides are peptides that bind exclusively to your concentrate on antibody and block antibody binding. These peptide generally is made up of the epitope regarded by the antibody.
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This guidebook outlines even more possible optimization of mobile and immunoassay parameters to make certain the best possible benefits are...
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This manual outlines further doable optimization of cellular and immunoassay parameters to ensure the very best benefits are...
Antibodies bound to the blocking peptide no more bind for the epitope over the goal protein. By evaluating the staining from your blocked antibody vs . the antibody by yourself, you can see which staining is particular.
Focus on Information Cyclin E belongs for the really conserved cyclin loved ones, whose customers are characterised by a dramatic periodicity in protein abundance with the mobile cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins show distinctive expression and degradation styles which add into the temporal coordination of each and every mitotic party. Cyclin E forms a posh with and capabilities to be a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S transition. This protein accumulates in the G1-S phase boundary and it is degraded as cells development as a result of S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by equally antibodies...
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